Help for the mirsnpscore database: SNP effects on microRNA targeting

This database provides computer-based predictions of SNP effects on microRNA-based gene regulation.

Search,Tutorial


Using the "Single search" form, one can search for:
- either a gene name (Ex: ADAR),
- or a refSeq Gene ID (Ex: NM_001111),
- or a human microRNA ID (Ex: hsa-miR-1285 or miR-1285),
- or a SNP ID (Ex: rs1127313).

The "Search type" field enables to select the kind of query (gene/miRNA/SNP).

One can limit the number of records shown in the output, by using the "Limit results to" option.


Using the "Multiple search" form, one can search at the same time for:
- a gene name,
- and a human microRNA ID,
- and a SNP ID.

The output shows then the records intersecting the different queries (gene,miRNA,SNP) if fieled. Here again, one can limit the number of records shown in the output, by using the "Limit results to" option.


The output starts by showing the query: which gene, miRNA, SNP, and the maximum number of records to show.

Column description:
- The first column shows the chromosome in the hg18 assembly.
- The second column shows the gene name, according to the RefSeq Gene database from UCSC.
- The third column shows the gene's RefSeq ID, according to the RefSeq database.
- The forth column shows the strand of the mRNA.
- The fifth column shows the human microRNA name according to miRBase database 16.
- The sixth column shows the predicted SNP effect, which is the difference of scores given by two haplotypes: DeltaS = |sc1 - sc2|
- The seventh column shows SNP IDs. They are located within the 3'UTR of the considered gene.
- The eighth column shows the SNP position in the hg18 assembly, based on HapMap database.
- The nineth column shows the minimum allele frequency (MAF) of the considerede SNP in the CEU Hapmap population.
- The tenth column shows the alleles of the considered SNP, based on HapMap database.
- The eleventh column shows the target site sequence (at the seed region) with the allele denoted within "[]" and the seed type.


Important comments

The first allele listed for a SNP is not necessarily the MAF allele, but the allele of the score sc1. The second allele is the allele of the score sc2.

If none of the allele of a given SNP provide target site sequence, it is either because the SNP affect 3'supplementary site, or because the SNP is strongly linked to another mirSNP in the same gene.

If two SNPs affect one target site in the seed region, with for instance:
- SNP1: "T" allele for score sc1 (that have the target site)
- SNP1: "C_T" alleles for score sc2 (that do not have the target site)
- SNP2: "A" allele for score sc1 (that have the target site)
- SNP2: "A_G" alleles for score sc2 (that do not have the target site)
It means that the haplotype TA has the target site and gives score s1, while any other combination (CA,TG,CG) has not the target site and give score sc2.

If looking for a gene and a SNP at the same time, the SNP has to lie within the 3'UTR of that gene.